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В данной работе описана CRISPR Cas система II-С типа, найденная в бактерии Clostridium cellulolyticum. Представлено описание основных компонентов системы: Cas генов и CRISPR кассеты. Определена РАМ последовательность, узнаваемая эффекторным белком CcCas9. Проверена активность системы CRISPR CcCas9 in vitro и в клетках человека.
This paper describes CRISPR Cas type II-С system found in the bacteria Clostridium cellulolyticum. The description of the main components of the system: Cas genes and CRISPR cassette is presented. The PAM sequence recognized by the effector protein CcCas9 was determined. The activity of the CRISPR CcCas9 system in vitro and in human cells was tested.
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