Детальная информация

This work is devoted to obtaining chondrogenically modified human dermal fibroblasts using protein-based induction and lentiviral delivery of SOX9- and TGFB3-carrying constructs. The relevance of the study is associated with the limited regenerative capacity of hyaline cartilage and the need to develop cell- based approaches for cartilage tissue engineering. Plasmid DNA isolation and quality control, restriction analysis, HEK293T transfection, lentiviral particle production, and dermal fibroblast transduction were performed. Modification efficiency was assessed using fluorescence microscopy, flow cytometry, RT-qPCR, and Alcian blue staining. GraphPad Prism 8, STATISTICA 10, ImageJ, and CyteExpert 2.3 software were used during the study. The results showed that human dermal fibroblasts can be modified using both protein-based and lentiviral approaches. Modified cells demonstrated changes in the expression of chondrogenesis-associated genes and accumulation of glycosaminoglycans in the extracellular matrix. The obtained data may be used for further development of scaffold-based tissue-engineered constructs for cartilage repair.