Детальная информация

The given work is devoted to editing the E. coli genome using the CRISPR- Cas system to produce a strain that expresses the FtsQ division apparatus protein and MinC protein fused with the mScarlet fluorescent protein, for their subsequent visualization in real-time in the cells. Using standard methods of molecular biology, we have created a construct containing the FtsQ protein of the cell division machinery and the fluorescent protein mScarlet. By using fluorescence microscopy, we found that the FtsQ protein colocalizes with FtsZ protein filaments forming a Z-ring. In the case of the Min-system component MinC, we attempted to create a fluorescent construct MinC::mScarlet, but this was unsuccessful due to difficulties in visualizing the construct. This may be due to construct’s toxicity, leading to the selective growth of clones without it. The importance of this research is undeniable, as the understanding of bacterial cell division mechanisms is both fundamental and applicable. Despite numerous studies, the process of bacterial cell division, including that of the model organism E. coli, remains incompletely understood. Proteins of the division machinery represent promising targets for developing novel antibacterial therapies.