Details

Title Механизмы ингибирования роста грамотрицательной бактериальной культуры E. coli белком бактериофага phiKZ Gp10: выпускная квалификационная работа магистра: направление 12.04.04 «Биотехнические системы и технологии» ; образовательная программа 12.04.04_01 «Молекулярные и клеточные биомедицинские технологии (международная образовательная программа) / Molecular and Cellular Biomedical Technologies (International Educational Program)»
Creators Сагайдак Екатерина
Scientific adviser Ведяйкин Алексей Дмитриевич
Organization Санкт-Петербургский политехнический университет Петра Великого. Институт биомедицинских систем и биотехнологий
Imprint Санкт-Петербург, 2025
Collection Выпускные квалификационные работы ; Общая коллекция
Subjects бактериофаг ; phiKZ ; ранние гены ; Escherichia coli ; антимикробные ; белки ; антибиотикорезистентность ; количественная ПЦР ; phiKZ bacteriophage ; early genes ; antimicrobial proteins ; antibiotic resistance ; qPCR
Document type Master graduation qualification work
File type PDF
Language Russian
Level of education Master
Speciality code (FGOS) 12.04.04
Speciality group (FGOS) 120000 - Фотоника, приборостроение, оптические и биотехнические системы и технологии
DOI 10.18720/SPBPU/3/2025/vr/vr25-2143
Rights Доступ по паролю из сети Интернет (чтение)
Additionally New arrival
Record key ru\spstu\vkr\35019
Record create date 7/4/2025

Allowed Actions

Action 'Read' will be available if you login or access site from another network

Group Anonymous
Network Internet

The given work is devoted to investigating the mechanisms of growth inhibition in the gram-negative bacterial culture Escherichia coli by the Gp10 protein of the phiKZ bacteriophage. The relevance of the topic is driven by the global problem of antibiotic resistance and the urgent need to discover new antimicrobial agents. The aim of the research was to identify the molecular mechanisms by which the Gp10 protein suppresses bacterial cell growth, with a particular focus on its potential impact on transcription and replication processes. The study employed molecular biology techniques, including Gp10 protein expression in E. coli cells, RNA and DNA extraction, cDNA synthesis, and quantitative PCR to assess the transcription level of the rpoB gene and analyze the oriC/ter DNA sequence ratio. The experiments revealed that the expression of the Gp10 protein disrupts replication processes, leading to the arrest of cell division. The obtained results may be applied to the searching for new antibiotic targets and the development of novel antimicrobial agents based on bacteriophage proteins, effective against antibiotic-resistant bacterial strains. The conclusions highlight the potential of the Gp10 protein as an agent that interferes with key processes essential for bacterial viability.

Network User group Action
ILC SPbPU Local Network All
Read
Internet Authorized users SPbPU
Read
Internet Anonymous

Access count: 0 
Last 30 days: 0

Detailed usage statistics