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Title | Механизмы ингибирования роста грамотрицательной бактериальной культуры E. coli белком бактериофага phiKZ Gp10: выпускная квалификационная работа магистра: направление 12.04.04 «Биотехнические системы и технологии» ; образовательная программа 12.04.04_01 «Молекулярные и клеточные биомедицинские технологии (международная образовательная программа) / Molecular and Cellular Biomedical Technologies (International Educational Program)» |
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Creators | Сагайдак Екатерина |
Scientific adviser | Ведяйкин Алексей Дмитриевич |
Organization | Санкт-Петербургский политехнический университет Петра Великого. Институт биомедицинских систем и биотехнологий |
Imprint | Санкт-Петербург, 2025 |
Collection | Выпускные квалификационные работы ; Общая коллекция |
Subjects | бактериофаг ; phiKZ ; ранние гены ; Escherichia coli ; антимикробные ; белки ; антибиотикорезистентность ; количественная ПЦР ; phiKZ bacteriophage ; early genes ; antimicrobial proteins ; antibiotic resistance ; qPCR |
Document type | Master graduation qualification work |
File type | |
Language | Russian |
Level of education | Master |
Speciality code (FGOS) | 12.04.04 |
Speciality group (FGOS) | 120000 - Фотоника, приборостроение, оптические и биотехнические системы и технологии |
DOI | 10.18720/SPBPU/3/2025/vr/vr25-2143 |
Rights | Доступ по паролю из сети Интернет (чтение) |
Additionally | New arrival |
Record key | ru\spstu\vkr\35019 |
Record create date | 7/4/2025 |
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The given work is devoted to investigating the mechanisms of growth inhibition in the gram-negative bacterial culture Escherichia coli by the Gp10 protein of the phiKZ bacteriophage. The relevance of the topic is driven by the global problem of antibiotic resistance and the urgent need to discover new antimicrobial agents. The aim of the research was to identify the molecular mechanisms by which the Gp10 protein suppresses bacterial cell growth, with a particular focus on its potential impact on transcription and replication processes. The study employed molecular biology techniques, including Gp10 protein expression in E. coli cells, RNA and DNA extraction, cDNA synthesis, and quantitative PCR to assess the transcription level of the rpoB gene and analyze the oriC/ter DNA sequence ratio. The experiments revealed that the expression of the Gp10 protein disrupts replication processes, leading to the arrest of cell division. The obtained results may be applied to the searching for new antibiotic targets and the development of novel antimicrobial agents based on bacteriophage proteins, effective against antibiotic-resistant bacterial strains. The conclusions highlight the potential of the Gp10 protein as an agent that interferes with key processes essential for bacterial viability.
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