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This paper studies the measurement of the electromotive force (zeta potential) of biocolloidal particles using the electrophoretic light scattering (ELS) technique. Several measurement methods are analyzed, including streaming potential, streaming current, and electroosmotic methods. Electrophoresis. Key factors such as sample purity, solution pH, ionic strength, and temperature are discussed, and their influence on the measurement accuracy is emphasized. The ELS method combines laser scattering with electrophoretic motion. The key devices of the experimental setup include a laser source, a beam splitter, a sample cell with electrodes, and a photodetector. The Doppler effect is used to measure the frequency shift of the scattered light caused by the motion of the particles under an applied electric field, and the electrophoretic mobility and zeta potential are calculated. The experimental study targets three colloidal systems: 100 nm latex microspheres, fullerol C60(OH)46, and bovine serum albumin (BSA). The results show that increasing the electric field strength does not significantly change the zeta potential, while adding electrolytes can enhance the electrokinetic potential in lyophilic systems such as BSA and fullerol. Studies on a 1:1 mixture of albumin and fullerol solutions show that when C60(OH)46 is added to the albumin solution, it destroys the aggregation stability of the system.

• CONTENTS
• CHAPTER 1. LITERATURE REVIEW
  • 1.1. Fluid
    • 1.1.1 Definition of fluid
    • 1.1.2 Fluid properties
  • 1.2 Colloid
    • 1.2.1 What is a colloid
    • 1.2.2 Properties of colloids
    • 1.2.3 Classification of colloids
  • 1.3 Electrophoresis and related knowledge
    • 1.3.1 Electrophoresis
    • 1.3.2 Electric field
    • 1.3.3 Solution pH
    • 1.3.4 Charge movement law
    • 1.3.5 Application fields
  • 1.4 Zeta potential
  • 1.5 Zeta potential measurement method
    • 1.5.1 Streaming potential method
    • 1.5.2 Flow current method
    • 1.5.3 Electroosmosis method
    • 1.5.4 Electrophoresis
    • 1.5.5 Laser Doppler electrophoresis method
    • 1.5.6 Comparison of the advantages and disadvantag
  • 1.6 Relationship between Zeta Potential and Electr
• CHAPTER 2. ELECTROPHORETIC LIGHT SCATTERING
  • 2.1 Basic principles of electrophoretic light scat
  • 2.2 Knowledge about electrophoretic light scatteri
    • 2.2.1 Sample requirements
    • 2.2.2 Solution-related requirements
  • 2.3 Basic scheme description of electrophoretic li
  • 2.4 Detailed functions of each part of the electro
    • 2.4.1 Laser
    • 2.4.2 Beam splitter
    • 2.4.3 Attenuator
    • 2.4.4 Sample pool
    • 2.4.5 Compensation optics
    • 2.4.6 Combined optics
    • 2.4.7 Detector
    • 2.4.8 Digital signal processor
    • 2.4.9 Computer
  • 2.5 Measurement and calculation of electrophoretic
  • 2.6 Correlation analysis
  • 2.7 Calculation of zeta potential
• CHAPTER 3. EXPERIMENTAL RESEARCH.
  • 3.1. Experimental design
    • 3.1.1 Introduction of some experimental instrument
    • 3.1.2 The function of each experimental equipment
  • 3.2. Testing of the laboratory setup
  • 3.3. Findings from investigations on colloidal sys
• REFERENCES