Детальная информация

The research is devoted to the analysis of the association between the content of the catalytic subunit of telomerase (TERT) and coilin protein in cumulus cells surrounding the oocyte and its fertilization ability, as well as embryo development to the blastocyst stage and its subsequent implantation. The study was performed using cumulus cell samples obtained from oocyte-cumulus complexes after stimulation of superovulation within ART programs. Immunohistochemical staining followed by fluorescence microscopy was implied to detect TERT and coilin. The obtained microphotographs were analyzed with ImageJ software. Statistical analysis was conducted using the Chi-square test and the Mann–Whitney U test. The results showed that the specific distribution of TERT and coilin, their spatial arrangement, the content of TERT and its connection to coilin were indeed associated with the formation of blastocyst and its implantation. The results obtained may be used for further investigation of additional predictors for assessing pre- and postimplantation embryo development in ART programs.