Details
| Title | Telomerase and coilin in cumulus cells as predictors of human oocyte competency for fertilization in vitro, pre- and postimplantation embryo development: выпускная квалификационная работа магистра: направление 12.04.04 «Биотехнические системы и технологии» ; образовательная программа 12.04.04_01 «Молекулярные и клеточные биомедицинские технологии (международная образовательная программа) / Molecular and Cellular Biomedical Technologies (International Educational Program)» |
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| Creators | Тестова Екатерина Сергеевна |
| Scientific adviser | Большакова Анастасия Викторовна |
| Organization | Санкт-Петербургский политехнический университет Петра Великого. Институт биомедицинских систем и биотехнологий |
| Imprint | Санкт-Петербург, 2026 |
| Collection | Выпускные квалификационные работы ; Общая коллекция |
| Subjects | cumulus cells ; TERT ; coilin ; oocyte ; blastocyst ; oocyte-cumulus complex ; implantation ; immunohistochemistry ; assisted reproductive technologies |
| Document type | Master graduation qualification work |
| Language | Russian |
| Level of education | Master |
| Speciality code (FGOS) | 12.04.04 |
| Speciality group (FGOS) | 120000 - Фотоника, приборостроение, оптические и биотехнические системы и технологии |
| DOI | 10.18720/SPBPU/3/2026/vr/vr26-4702 |
| Rights | Доступ по паролю из сети Интернет (чтение) |
| Additionally | New arrival |
| Record key | ru\spstu\vkr\45228 |
| Record create date | 9/7/2026 |
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| Group | Anonymous |
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| Network | Internet |
The research is devoted to the analysis of the association between the content of the catalytic subunit of telomerase (TERT) and coilin protein in cumulus cells surrounding the oocyte and its fertilization ability, as well as embryo development to the blastocyst stage and its subsequent implantation. The study was performed using cumulus cell samples obtained from oocyte-cumulus complexes after stimulation of superovulation within ART programs. Immunohistochemical staining followed by fluorescence microscopy was implied to detect TERT and coilin. The obtained microphotographs were analyzed with ImageJ software. Statistical analysis was conducted using the Chi-square test and the Mann–Whitney U test. The results showed that the specific distribution of TERT and coilin, their spatial arrangement, the content of TERT and its connection to coilin were indeed associated with the formation of blastocyst and its implantation. The results obtained may be used for further investigation of additional predictors for assessing pre- and postimplantation embryo development in ART programs.
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| ILC SPbPU Local Network | All |
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| Internet | Authorized users SPbPU |
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| Internet | Anonymous |
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