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This study aims to systematically investigate the key factors affecting the output performance of the preamplifier, reveal the influence mechanisms of various parameters on pump conversion efficiency and gain characteristics, and provide experimental basis and theoretical reference for the design optimization and performance improvement of the preamplification stage in high-power polarization-maintaining pulsed MOPA systems. This work aims to build an all-polarization-maintaining ytterbium-doped panda fiber preamplifier experimental platform and analyze the influence laws of various key parameters on the amplifier performance through experimental measurements. To achieve this goal, it is necessary to complete the assembly and debugging of the all-polarization-maintaining fiber optical path, systematically study the effects of seed optical power, pump optical power and active fiber length on the output amplified signal power and residual pump power, calculate the conversion efficiency of pump power to amplified signal power, and determine the preferable operating interval of the preamplifier within the parameter scope of the current study through experimental data analysis. This study experimentally verifies the feasibility of the all-polarization-maintaining ytterbium-doped panda fiber preamplifier at 1040 nm band, quantifies the influence degree of each parameter on the amplifier performance, and provides experimental basis and technical support for the overall design, optimization and engineering application of subsequent high-power polarization-maintaining pulsed fiber lasers.