Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.

• Preface
• Contents
• Introducing the Bacterial Antibiotic Sensor Mini Project
• Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are I
• Designing Primers for Site-Directed Mutagenesis
• Performing Site-Directed Mutagenesis
• Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel
• Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
• Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
• Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV s
• Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluo
• Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
• Understanding the Importance of Buffers in Biological Systems
• Molecular Visualization of an Enzyme, Acetylcholinesterase
• Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experimen
• Separation of the Phosphatidylcholines Using Reverse Phase HPLC
• List of Figures