Details
| Title | Biochemistry Laboratory Manual For Undergraduates. |
|---|---|
| Creators | Fernandez Timea Gerczei |
| Other creators | Scott Pattison |
| Organization | TotalBoox, ; TBX, |
| Imprint | De Gruyter, 2015 |
| Collection | Электронные книги зарубежных издательств ; Общая коллекция |
| Subjects | Biochemistry — Laboratory manuals. ; Molecular biology — Laboratory manuals. ; Drug resistance in microorganisms — Laboratory manuals. ; EBSCO eBooks |
| Document type | Other |
| File type | |
| Language | English |
| Rights | Доступ по паролю из сети Интернет (чтение, печать, копирование) |
| Record key | ocn969017645 |
| Record create date | 3/11/2015 |
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Biochemistry Laboratory Manual for undergraduates is the first textbook on the market that uses a highly relevant model, antibiotic resistance, to teach seminal topics of biochemistry and molecular biology. Inclusion of a research project does not entail a limitation: this manual includes all classic biochemistry techniques such as HPLC or enzyme kinetics and is complete with numerous problem sets relating to each topic.
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- Preface
- Contents
- Introducing the Bacterial Antibiotic Sensor Mini Project
- Identifying Conserved Elements in the Toxin Sensor and Designing Mutants to Test Whether They are I
- Designing Primers for Site-Directed Mutagenesis
- Performing Site-Directed Mutagenesis
- Purifying Mutant Toxin Sensor DNA from Bacterial Cells and Evaluating its Quality Using Agarose Gel
- Preparing DNA Template for Mutant RNA Sensor Synthesis Using a Restriction Endonuclease
- Synthesizing the ykkCD Mutant Toxin Sensor RNA in vitro
- Purifying the ykkCD Mutant Toxin Sensor RNA and Evaluating its Purity Using Denaturing PAGE and UV s
- Evaluating the Ability of the ykkCD Toxin Sensor to Recognize the Antibiotic Tetracycline Using Fluo
- Evaluating Antibiotic Binding to Blood Serum Albumin Using Fluorescence Spectroscopy
- Understanding the Importance of Buffers in Biological Systems
- Molecular Visualization of an Enzyme, Acetylcholinesterase
- Determining the Efficiency of the Enzyme Acetylcholine Esterase Using Steady-State Kinetic Experimen
- Separation of the Phosphatidylcholines Using Reverse Phase HPLC
- List of Figures
